Drosophila S2 cells are non-permissive for vaccinia virus DNA replication following entry via low pH-dependent endocytosis and early transcription.
Vaccinia virus (VACV), a member of the chordopox subfamily of the Poxviridae, abortively infects insect cells.We have investigated VACV infection of Drosophila S2 cells, which are useful for protein expression and genome-wide RNAi screening.Biochemical and electron microscopic analyses indicated that VACV entry into Drosophila S2 cells depended on